Three recent papers from the group
Extending single-cell proteomics from HeLa to small primary human immune cells
A high-sensitivity, label-free single-cell proteomics platform that combines optimised low-input sample processing, refined liquid chromatography and the Orbitrap Astral Zoom. It quantifies up to 7,000 proteins per HeLa cell at over 100 samples per day, currently a record for single-cell proteomics, and up to 4,000 proteins in small primary immune cells, enough to distinguish monocytes, T cells and activated lymphocytes.
Read the paper →Global analysis of protein turnover dynamics in single cells
Single-cell proteomics has mostly measured how much of each protein a cell contains. Using pulsed SILAC (SC-pSILAC), this study measures protein abundance and turnover together, detecting both labels on around 4,000 proteins in single HeLa cells. Across more than 1,000 cells from differentiating stem cells, turnover dynamics separated dividing from non-dividing cells and revealed co-regulated protein complexes.
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Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics
Chip-Tip is a high-sensitivity, label-free workflow that identifies more than 5,000 proteins in individual HeLa cells at up to 120 samples per day. It detects post-translational modifications directly, without enrichment, and in differentiating stem cells it quantified pluripotency markers such as OCT4 and SOX2 alongside lineage markers for all three germ layers.
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