Three recent papers from the group
One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes
One-Tip combines one-pot sample preparation in a single pipette tip with narrow-window DIA, removing the multi-step processing that causes sample loss. It identifies more than 9,000 proteins from around 1,000 HeLa cells, around 6,000 in single cells from early mouse embryos, and more than 3,000 from just 16 ng of plasma extracellular vesicles.
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Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition
Narrow-window DIA (nDIA) pairs a quadrupole Orbitrap with the Astral analyzer to run MS/MS scans at around 200 Hz with 2-Th windows, closing the gap between data-dependent and data-independent methods. It reaches around 10,000 human protein groups in half an hour, or around 7,000 in five minutes, about three times the coverage of earlier state-of-the-art MS, and quantifies more than 14,000 protein groups in a single 30-minute run.
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Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution
A high-throughput workflow based on sequential cell fractionation that profiles the proteome and phosphoproteome across six subcellular fractions. Benchmarked on EGFR signalling in HeLa cells and mouse tissues, it also revealed the relocation of ribosomal proteins in response to hypertonic stress and muscle contraction. The data can be explored at SpatialProteoDynamics.github.io.
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